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Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13

Abstract: The anaphase-promoting complex/cyclosome (APC/C) is a 22S ubiquitin ligase complex that initiates chromosome segregation and mitotic exit. We have used biochemical and electron microscopic analyses of Saccharomyces cerevisiae and human APC/C to address how the APC/C subunit Doc1 contributes to recruitment and processive ubiquitylation of APC/C substrates, and to understand how APC/C monomers interact to form a 36S dimeric form. We show that Doc1 interacts with Cdc27, Cdc16 and Apc1 and is located in the vicinity of the cullin-RING module Apc2-Apc11 in the inner cavity of the APC/C. Substrate proteins also bind in the inner cavity, in close proximity to Doc1 and the coactivator Cdh1, and induce conformational changes in Apc2-Apc11. Our results suggest that substrates are recruited to the APC/C by binding to a bipartite substrate receptor composed of a coactivator protein and Doc1.

Status: Published Type: Journal Article PubMed ID: 21186364

Topics addressed in this paper

Number of different genes curated to this paper: 14

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Topics Genes linked to topics (#1 - 10 )
APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC27 CDH1
Additional Literature blue ball blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#11 - 14 )
DOC1 HSL1 MND2 SWM1
Additional Literature blue ball
Cellular Location blue ball
Mutants/Phenotypes blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball
Primary Literature blue ball blue ball blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball

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