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Lockhart L, et al.  (2002) Tools for the study of genome rearrangements in laboratory and industrial yeast strains. Yeast 19(5):441-8

Abstract: In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations.CI - Copyright 2002 John Wiley & Sons, Ltd.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 11921092

Topics addressed in this paper

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ADH4 ALR2 ATF1 BIO3 BIO4 CAN1 CBP1 CBP2 CDC10 CDC13
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CDC39 CDC5 CHA1 DEG1 DMC1 DNM1 FPS1 GDH3 HAT1 HMG2
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HTA2 INP51 LYS1 MCD1 MET14 MNN1 PKC1 PLC1 PMA1 PMT4
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Topics Genes linked to topics (#31 - 40 )
RFA2 RIF1 RLP7 SAG1 SEC23 SIN3 SIR1 SKN7 SMC2 SPS1
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SPT20 STE20 TFC3 TUB3 UBP7 URA1 YAR044W YME2 YOR1
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