SGD Paper Help


Page Contents:
Kingston IJ, et al.  (2011) Biophysical Characterization of the Centromere-specific Nucleosome from Budding Yeast. J Biol Chem 286(5):4021-6

Abstract: The centromeric DNA of all eukaryotes is assembled upon a specialised nucleosome containing a histone H3 variant known as CenH3. Despite the importance and conserved nature of this protein, the characteristics of the centromeric nucleosome are still poorly understood. In particular, the stoichiometry and DNA-binding properties of the CenH3 nucleosome have been the subject of some debate. We have characterised the budding yeast centromeric nucleosome by biochemical and biophysical methods and show that it forms a stable octamer containing two copies of the Cse4 protein, and wraps DNA in a left-handed supercoil, similar to the canonical H3 nucleosome. The DNA-binding properties of the recombinant nucleosome are identical to those observed in vivo demonstrating that the octameric structure is physiologically relevant.

Status: Published Type: Journal Article PubMed ID: 21115484

Topics addressed in this paper

Number of different genes curated to this paper: 7

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
CSE4 HHF1 HHF2 HHT1 HHT2 HTB1 HTB2
Fungal Related Genes/Proteins blue ball
Mutants/Phenotypes blue ball
Non-Fungal Related Genes/Proteins blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: