Duch A, et al. (2011) A Dbf4 Mutant Contributes to Bypassing the Rad53-mediated Block of Origins of Replication in Response to Genotoxic Stress. J Biol Chem 286(4):2486-91
Abstract: An intra-S phase checkpoint slows the rate of DNA replication in response to DNA damage and replication fork blocks in eukaryotic cells. In the budding yeast Saccharomyces cerevisiae such down-regulation is achieved through the Rad53 kinase dependent block of origins of replication. We have identified the Rad53 phosphorylation sites on Dbf4, the activator subunit of the essential S-phase kinase DDK (Dbf4 Dependent Kinase), and generated an unphosphorylatable Dbf4 mutant (dbf4(7A)). We show here that dbf4(7A) is a bona fide intra-S phase checkpoint bypass allele, that contributes to abrogate the Rad53 block of origin firing in response to genotoxic stress.
|Status: Published||Type: Journal Article||PubMed ID: 21098477|
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