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Soboleva NG, et al.  (2003) [The effect of modification of nucleotide-37 on the interaction of aminoacyl-tRNA with the A-site of the 70S ribosome] Mol Biol (Mosk) 37(1):121-7

Abstract: To estimate the effect of modified nucleotide-37, the interaction of two yeast aminoacyl-tRNAs (Phe-tRNAK+YPhe and Phe-tRNAK-YPhe) with the A site of complex [70S.poly(U).deacylated tRNA(Phe) in the P site] was assayed at 0-20 degrees C. As comparisons with native Phe-tRNAK+YPhe showed, removal of the Y base decreased the association constant of Phe-tRNAK-YPhe and the complex by an order of magnitude at any temperature, and increased the enthalpy of their interaction by 23 kJ/mol. When the Y base was present in the anticodon loop of deacylated tRNA(Phe) bound to the P site of the 70S ribosome, twice higher affinity for the A site was observed for Phe-tRNAK-YPhe but not for Phe-tRNAK+YPhe. Thus, the modified nucleotide 3' of the Phe-tRNA(Phe) anticodon stabilized the codon-anticodon interaction both in the A and in the P sites of the 70S ribosome.

Status: Published Type: English Abstract | Journal Article PubMed ID: 12624954

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tF(GAA)B tF(GAA)D tF(GAA)F tF(GAA)G tF(GAA)H1 tF(GAA)H2 tF(GAA)M tF(GAA)N tF(GAA)P1 tF(GAA)P2
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Topics Genes linked to topics (#11 )
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