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Denison C and Kodadek T  (2004) Toward a general chemical method for rapidly mapping multi-protein complexes. J Proteome Res 3(3):417-25

Abstract: Ru(II)(bpy2)32+Cl2, ammonium persulfate, and visible light irradiation has been shown to rapidly and efficiently cross-link several interacting proteins. However, this methodology has not yet been used to map the architecture of large multi-protein complexes. In this study, this chemistry is applied to the crystallographically characterized yeast proteasome. The data obtained demonstrate both the method's increased generality and fidelity in comparison to traditional bifunctional cross-linking reagents, while also highlighting the future need for developing better analytical techniques to separate cross-linked products.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S. PubMed ID: 15253422

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Topics Genes linked to topics (#1 - 10 )
BLM10 CIC1 DOA4 ECM29 HUL5 NAS6 PRE1 PRE10 PRE2 PRE3
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP1 PUP2 PUP3 RAD23
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
RAD6 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN3 RPN5
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 47 )
SCL1 SEM1 UBC1 UBC4 UBC5 UBP6 UMP1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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