Sari MM (2011) Investigation of Yeast Invertase Immobilization onto Cupric Ion-Chelated, Porous, and Biocompatible Poly(Hydroxyethyl Methacrylate-n-Vinyl Imidazole) Microspheres. Appl Biochem Biotechnol 163(8):1020-37
Abstract: Cupric ion-chelated poly(hydroxyethyl methacrylate-n-vinyl imidazole) (poly(HEMA-VIM)) microspheres prepared by suspension polymerization were investigated as a specific adsorbent for immobilization of yeast invertase in a batch system. They were characterized by scanning electron microscopy, surface area, and pore size measurements. They have spherical shape and porous structure. The specific surface area of the p(HEMA-VIM) spheres was found to be 81.2 m(2)/g with a size range of 70-120 mum in diameter, and the swelling ratio was 86.9%. Then, Cu(II) ion chelated on the microspheres (546 mumol Cu(II)/g), and they were used in the invertase adsorption. Maximum invertase adsorption was 51.2 mg/g at pH 4.5. Cu(II) chelation increases the tendency from Freundlich-type to Langmuir-type adsorption model. The optimum activity for both free and adsorbed invertase was observed at pH 4.5. The optimum temperature for the poly(HEMA-VIM)/Cu(II)-invertase system was found to be at 55 degrees C, 10 degrees C higher than that of the free enzyme at 45 degrees C. V (max) values were determined as 342 and 304 U/mg enzyme, for free and adsorbed invertase, respectively. K (m) values were found to be same for free and adsorbed invertase (20 mM). Thermal and pH stability and reusability of invertase increased with immobilization.
|Status: Published||Type: Journal Article||PubMed ID: 20972892|
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