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Vannini A, et al.  (2010) Molecular basis of RNA polymerase III transcription repression by Maf1. Cell 143(1):59-70

Abstract: RNA polymerase III (Pol III) transcribes short RNAs required for cell growth. Under stress conditions, the conserved protein Maf1 rapidly represses Pol III transcription. We report the crystal structure of Maf1 and cryo-electron microscopic structures of Pol III, an active Pol III-DNA-RNA complex, and a repressive Pol III-Maf1 complex. Binding of DNA and RNA causes ordering of the Pol III-specific subcomplex C82/34/31 that is required for transcription initiation. Maf1 binds the Pol III clamp and rearranges C82/34/31 at the rim of the active center cleft. This impairs recruitment of Pol III to a complex of promoter DNA with the initiation factors Brf1 and TBP and thus prevents closed complex formation. Maf1 does however not impair binding of a DNA-RNA scaffold and RNA synthesis. These results explain how Maf1 specifically represses transcription initiation from Pol III promoters and indicate that Maf1 also prevents reinitiation by binding Pol III during transcription elongation.CI - Copyright (c) 2010 Elsevier Inc. All rights reserved.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 20887893

Topics addressed in this paper

Number of different genes curated to this paper: 20

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Topics Genes linked to topics (#1 - 10 )
BRF1 MAF1 RET1 RPB10 RPB5 RPB8 RPC10 RPC11 RPC17 RPC19
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Topics Genes linked to topics (#11 - 20 )
RPC25 RPC31 RPC34 RPC37 RPC40 RPC53 RPC82 RPO26 RPO31 SPT15
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Regulation of blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

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