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Fekete CA, et al.  (2005) The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo. EMBO J 24(20):3588-601

Abstract: Translation initiation factor 1A stimulates 40S-binding of the eukaryotic initiation factor 2 (eIF2)/GTP/Met-tRNA(iMet) ternary complex (TC) and promotes scanning in vitro. eIF1A contains an OB-fold present in bacterial IF1 plus N- and C-terminal extensions. Truncating the C-terminus (deltaC) or mutating OB-fold residues (66-70) of eIF1A reduced general translation in vivo but increased GCN4 translation (Gcd- phenotype) in a manner suppressed by overexpressing TC. Consistent with this, both mutations diminished 40S-bound TC, eIF5 and eIF3 in vivo, and deltaC impaired TC recruitment in vitro. The assembly defects of the OB-fold mutation can be attributed to reduced 40S-binding of eIF1A, whereas deltaC impairs eIF1A function on the ribosome. A substitution in the C-terminal helix (98-101) also reduced 43S assembly in vivo. Rather than producing a Gcd- phenotype, however, 98-101 impairs GCN4 derepression in a manner consistent with defective scanning by reinitiating ribosomes. Indeed, 98-101 allows formation of aberrant 48S complexes in vitro and increases utilization of non-AUG codons in vivo. Thus, the OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in TC recruitment and scanning.

Status: Published Type: Journal Article PubMed ID: 16193068

Topics addressed in this paper

Number of different genes curated to this paper: 79

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Topics Genes linked to topics (#1 - 10 )
ASC1 CLU1 ENP1 GCD11 HCR1 LTV1 NIP1 NOB1 NOP9 PRT1
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Topics Genes linked to topics (#11 - 20 )
RDN18-1 RDN18-2 RPG1 RPS0A RPS0B RPS10A RPS10B RPS11A RPS11B RPS12
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Topics Genes linked to topics (#21 - 30 )
RPS13 RPS14A RPS14B RPS15 RPS16A RPS16B RPS17A RPS17B RPS18A RPS18B
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Topics Genes linked to topics (#31 - 40 )
RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B
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Topics Genes linked to topics (#41 - 50 )
RPS23A RPS23B RPS24A RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B
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Topics Genes linked to topics (#51 - 60 )
RPS28A RPS28B RPS29A RPS29B RPS3 RPS30A RPS30B RPS31 RPS4A RPS4B
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Topics Genes linked to topics (#61 - 70 )
RPS5 RPS6A RPS6B RPS7A RPS7B RPS8A RPS8B RPS9A RPS9B RRP1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#71 - 79 )
SLX9 SQS1 SUI2 TIF11 TIF34 TIF35 TIF5 UTP10 UTP20
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Function/Process blue ball
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