SGD Paper 
Weber M, et al. (2010) Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1. Mol Biol Cell 21(8):1362-74
Abstract: Sec1p/Munc18 (SM) family proteins regulate SNARE complex function in membrane fusion through their interactions with syntaxins. In addition to syntaxins, only a few SM protein interacting proteins are known and typically, their binding modes with SM proteins are poorly characterized. We previously identified Mso1p as a Sec1p-binding protein and showed that it is involved in membrane fusion regulation. Here we demonstrate that Mso1p and Sec1p interact at sites of exocytosis and that the Mso1p-Sec1p interaction site depends on a functional Rab GTPase Sec4p and its GEF Sec2p. Random and targeted mutagenesis of Sec1p, followed by analysis of protein interactions, indicates that Mso1p interacts with Sec1p domain 1 and that this interaction is important for membrane fusion. In many SM family proteins, domain 1 binds to a N-terminal peptide of a syntaxin family protein. The Sec1p-interacting syntaxins Sso1p and Sso2p lack the N-terminal peptide. We show that the putative N-peptide binding area in Sec1p domain 1 is important for Mso1p binding, and that Mso1p can interact with Sso1p and Sso2p. Our results suggest that Mso1p mimics N-peptide binding to facilitate membrane fusion.
| Status: Published | Type: Journal Article | Research Support, Non-U.S. Gov't | PubMed ID: 20181830 |
Topics addressed in this paper
Number of different genes curated to this paper: 10
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.
| Topics | Genes linked to topics | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MSO1 | SEC1 | SEC15 | SEC18 | SEC2 | SEC4 | SEC6 | SEC9 | SSO1 | SSO2 | |
| Cellular Location |  | | | | ||||||
| Mutants/Phenotypes | | | | | | | | | ||
| Primary Literature | | | | | | | | | | |
| Protein Processing/Modification/Regulation | | |||||||||
| Protein Sequence Features | | |||||||||
| Protein-protein Interactions | | | | | ||||||
| Protein/Nucleic Acid Structure | | |||||||||
| Regulation of | | |||||||||
| Regulatory Role | | |||||||||
| Strains/Constructs | | | | | | | | | | |
