SGD Paper 
Raab AM, et al. (2010) Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid. Metab Eng 12(6):518-25
Abstract: The production of bio-based succinic acid is receiving great attention, and several predominantly prokaryotic organisms have been evaluated for this purpose. In this study we report on the suitability of the highly acid- and osmotolerant yeast Saccharomyces cerevisiae as a succinic acid production host. We implemented a metabolic engineering strategy for the oxidative production of succinic acid in yeast by deletion of the genes SDH1, SDH2, IDH1, and IDP1. The engineered strains harbour a TCA cycle that is completely interrupted after the intermediates isocitrate and succinate. The strains show no serious growth constraints on glucose. In glucose-grown shake flask cultures, the quadruple deletion strain Deltasdh1Deltasdh2Deltaidh1Deltaidp1 produces succinic acid at a titer of 3.62g L(-1) (factor 4.8 compared to wild-type) at a yield of 0.11mol (mol glucose)(-1). Succinic acid is not accumulated intracellularly. This makes the yeast Saccharomyces cerevisiae a suitable and promising candidate for the biotechnological production of succinic acid on an industrial scale.CI - Copyright (c) 2010. Published by Elsevier Inc.
| Status: Published | Type: Journal Article | PubMed ID: 20854924 |
Topics addressed in this paper
Number of different genes curated to this paper: 4
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.
