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Wisselink HW, et al.  (2010) Metabolome, transcriptome and metabolic flux analysis of arabinose fermentation by engineered Saccharomyces cerevisiae. Metab Eng 12(6):537-51

Abstract: One of the challenges in strain improvement by evolutionary engineering is to subsequently determine the molecular basis of the improved properties that were enriched from the natural genetic variation during the selective conditions. This study focuses on Saccharomyces cerevisiae IMS0002 which, after metabolic and evolutionary engineering, ferments the pentose sugar arabinose. Glucose and arabinose-limited anaerobic chemostat cultures of IMS0002 and its non-evolved ancestor were subjected to transcriptome analysis, intracellular metabolite measurements and metabolic flux analysis. Increased expression of the GAL-regulon and deletion of GAL2 in IMS0002 confirmed that the galactose transporter is essential for growth on arabinose. Elevated intracellular concentrations of pentose-phosphate-pathway intermediates and upregulation of TKL2 and YGR043c (encoding transketolase and transaldolase isoenzymes) suggested an involvement of these genes in flux-controling reactions in arabinose fermentation. Indeed, deletion of these genes in IMS0002 caused a 21 % reduction of the maximum specific growth rate on arabinose.CI - Copyright (c) 2010. Published by Elsevier Inc.

Status: Published Type: Journal Article PubMed ID: 20816840

Topics addressed in this paper

Number of different genes curated to this paper: 43

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ADH2 AGA1 AGA2 ALD4 ALD6 BAR1 CIT3 DAK2 FBP1 FUS1
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Cell Growth and Metabolism yg ball
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Topics Genes linked to topics (#11 - 20 )
FUS3 GAL1 GAL10 GAL2 GAL3 GAL7 GAL80 GCY1 GND2 GPD1
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Topics Genes linked to topics (#21 - 30 )
GUT2 ICL1 IDH1 IDP2 KGD1 KGD2 LSC2 MFA1 MFA2 MLF3
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Topics Genes linked to topics (#31 - 40 )
MLS1 MSK1 NQM1 PCL10 RIO1 SDH1 SDH3 STE6 TDH1 TEC1
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Topics Genes linked to topics (#41 - 43 )
TKL2 YEL057C YPR1
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