Yagi H, et al. (2010) Tunable paramagnetic relaxation enhancements by [Gd(DPA)(3)] (3-) for protein structure analysis. J Biomol NMR 47(2):143-53
Abstract: Paramagnetic relaxation enhancements (PRE) present a powerful source of structural information in nuclear magnetic resonance (NMR) studies of proteins and protein-ligand complexes. In contrast to conventional PRE reagents that are covalently attached to the protein, the complex between gadolinium and three dipicolinic acid (DPA) molecules, [Gd(DPA)(3)](3-), can bind to proteins in a non-covalent yet site-specific manner. This offers straightforward access to PREs that can be scaled by using different ratios of [Gd(DPA)(3)](3-) to protein, allowing quantitative distance measurements for nuclear spins within about 15 A of the Gd(3+) ion. Such data accurately define the metal position relative to the protein, greatly enhancing the interpretation of pseudocontact shifts induced by [Ln(DPA)(3)](3-) complexes of paramagnetic lanthanide (Ln(3+)) ions other than gadolinium. As an example we studied the quaternary structure of the homodimeric GCN4 leucine zipper.
|Status: Published||Type: Journal Article | Research Support, Non-U.S. Gov't||PubMed ID: 20405166|
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|Protein Sequence Features|
|Protein/Nucleic Acid Structure|
|Techniques and Reagents|