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Nanda JS, et al.  (2009) eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation. J Mol Biol 394(2):268-85

Abstract: Eukaryotic translation initiation factor (eIF) 1 is a central mediator of start codon recognition. Dissociation of eIF1 from the preinitiation complex (PIC) allows release of phosphate from the G-protein factor eIF2, triggering downstream events in initiation. Mutations that weaken binding of eIF1 to the PIC decrease the fidelity of start codon recognition (Sui(-) phenotype) by allowing increased eIF1 release at non-AUG codons. Consistent with this, overexpression of these mutant proteins suppresses their Sui(-) phenotypes. Here, we have examined mutations at the penultimate residue of eIF1, G107, that produce Sui(-) phenotypes without increasing the rate of eIF1 release. We provide evidence that, in addition to its role in gating phosphate release, dissociation of eIF1 triggers conversion from an open, scanning-competent state of the PIC to a stable, closed one. We also show that eIF5 antagonizes binding of eIF1 to the complex and that key interactions of eIF1 with its partners are modulated by the charge at and around G107. Our data indicate that eIF1 plays multiple roles in start codon recognition and suggest that prior to AUG recognition it prevents eIF5 from binding to a key site in the PIC required for triggering downstream events.

Status: Published Type: Journal Article | Research Support, N.I.H., Extramural | Research Support, N.I.H., Intramural PubMed ID: 19751744

Topics addressed in this paper

Number of different genes curated to this paper: 71

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Topics Genes linked to topics (#1 - 10 )
ASC1 GCD2 NIP1 PRT1 RDN18-1 RDN18-2 RPG1 RPS0A RPS0B RPS10A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#11 - 20 )
RPS10B RPS11A RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16A RPS16B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
RPS17A RPS17B RPS18A RPS18B RPS19A RPS19B RPS1A RPS1B RPS2 RPS20
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RPS21A RPS21B RPS22A RPS22B RPS23A RPS23B RPS24A RPS24B RPS25A RPS25B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 50 )
RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#51 - 60 )
RPS30B RPS31 RPS4A RPS4B RPS5 RPS6A RPS6B RPS7A RPS7B RPS8A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
RPS8B RPS9A RPS9B SUI1 SUI3 SUP35 SUP45 TIF11 TIF34 TIF35
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball
Protein Physical Properties blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball
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Techniques and Reagents blue ball blue ball

Topics Genes linked to topics (#71 )
TIF5
Function/Process blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball
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