SGD Paper 
Page Contents:
Charmantray F, et al. (2010) Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone--Development, scope and limitations. J Biotechnol 145(4):359-66
Abstract: 5-O-Coumarinyl-d-xylulose was studied as a fluorogenic substrate for the stereospecific assay of transketolase enzyme. Enzymatic C2-C3 cleavage released an alpha-hydroxyl, beta-coumarinyl substituted aldehyde. Although the subsequent beta-elimination step was rate limiting under chemical or enzymatic catalysis, we detected a TK activity as low as 0.7mIU. To improve the fluorescence signal release, kinetic and product distribution analyses of this reaction were performed by LC/UV/MS coupling.CI - Copyright 2010 Elsevier B.V. All rights reserved.
| Status: Published | Type: Journal Article | PubMed ID: 20074593 |
Topics addressed in this paper
Number of different genes curated to this paper: 2
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.
Page Contents:
