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Tsakraklides V and Bell SP  (2010) Dynamics of pre-replicative complex assembly. J Biol Chem 285(13):9437-43

Abstract: The pre-replicative complex (pre-RC) is formed at all potential origins of replication through the action of the origin recognition complex (ORC), Cdc6, Cdt1, and the Mcm2-7 complex. The end result of pre-RC formation is the loading of the Mcm2-7 replicative helicase onto origin DNA. We examined pre-RC formation in vitro and found that it proceeds through separable binding events. Origin-bound ORC recruits Cdc6, and this ternary complex then promotes helicase loading in the presence of a pre-formed Mcm2-7-Cdt1 complex. Using a stepwise pre-RC assembly assay, we investigated the fate of pre-RC components during later stages of the reaction. We determined that helicase loading is accompanied by dissociation of ORC, Cdc6, and Cdt1 from origin DNA. This dissociation requires ATP hydrolysis at a late stage of pre-RC assembly. Our results indicate that pre-RC formation is a dynamic process.

Status: Published Type: Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't PubMed ID: 20097898

Topics addressed in this paper

Number of different genes curated to this paper: 14

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Topics Genes linked to topics (#1 - 10 )
CDC6 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3
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Topics Genes linked to topics (#11 - 14 )
ORC4 ORC5 ORC6 TAH11
Alias blue ball
Function/Process blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball
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