SGD Paper Help


Page Contents:
Mishra A, et al.  (2010) Both interaction surfaces within cohesin's hinge domain are essential for its stable chromosomal association. Curr Biol 20(4):279-89

Abstract: BACKGROUND: The cohesin complex that mediates sister chromatid cohesion contains three core subunits: Smc1, Smc3, and Scc1. Heterotypic interactions between Smc1 and Smc3 dimerization domains create stable V-shaped Smc1/Smc3 heterodimers with a hinge at the center and nucleotide-binding domains (NBDs) at the ends of each arm. Interconnection of each NBD through their association with the N- and C-terminal domains of Scc1 creates a tripartite ring, within which sister DNAs are thought to be entrapped (the ring model). Crystal structures show that the Smc1/Smc3 hinge has a toroidal shape, with independent "north" and "south" interaction surfaces on an axis of pseudosymmetry. The ring model predicts that sister chromatid cohesion would be lost by transient hinge opening. RESULTS: We find that mutations within either interface weaken heterodimerization of isolated half hinges in vitro but do not greatly compromise formation of cohesin rings in vivo. They do, however, reduce the residence time of cohesin on chromosomes and cause lethal defects in sister chromatid cohesion. This demonstrates that mere formation of rings is insufficient for cohesin function. Stable cohesion requires cohesin rings that cannot easily open. CONCLUSIONS: Either the north or south hinge interaction surface is sufficient for the assembly of V-shaped Smc1/Smc3 heterodimers in vivo. Any tendency of Smc proteins with weakened hinges to dissociate will be suppressed by interconnection of their NBDs by Scc1. We suggest that transient hinge dissociation caused by the mutations described here is incompatible with stable sister chromatid cohesion because it permits chromatin fibers to escape from cohesin rings.CI - Copyright 2010 Elsevier Ltd. All rights reserved.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 20153193

Topics addressed in this paper

Number of different genes curated to this paper: 3

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
MCD1 SMC1 SMC3
Alias blue ball
Cellular Location blue ball blue ball
Function/Process blue ball blue ball
Mutants/Phenotypes blue ball blue ball
Non-Fungal Related Genes/Proteins blue ball blue ball
Primary Literature blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball
Protein/Nucleic Acid Structure blue ball blue ball
Strains/Constructs blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: