Rogoza T, et al. (2010) Non-Mendelian determinant [ISP+] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1. Proc Natl Acad Sci U S A 107(23):10573-7
Abstract: Four protein-based genetic determinants or prions-[SWI (+)], [MCA], [OCT (+)], and [MOT3(+) ]-are recent additions to the list of well-known Saccharomyces cerevisiae prions, [PSI (+)], [URE3], and [PIN (+)]. A rapid expansion of this list may indicate that many yeast proteins can convert into heritable prion forms and underscores a problem of prion input into cellular physiology. Here, we prove that the global transcriptional regulator Sfp1 can become a prion corresponding to the prion-like determinant [ISP (+)] described earlier. We show that SFP1 deletion causes an irreversible [ISP (+)] loss, whereas increased SFP1 expression induces [ISP (+)] appearance. Cells that display the [ISP (+)] phenotype contain the aggregated form of Sfp1. Indeed, these aggregates demonstrate a nuclear location. We also show that the phenotypic manifestation of Sfp1 prionization differs from the manifestation of SFP1 deletion. These properties and others distinguish [ISP (+)] from yeast prions described to date.
|Status: Published||Type: Journal Article||PubMed ID: 20498075|
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