SGD Paper Help


Page Contents:
Spedale G, et al.  (2010) Identification of Pep4p as the protease responsible for formation of the SAGA-related SLIK protein complex. J Biol Chem 285(30):22793-9

Abstract: The Saccharomyces cerevisiae SAGA protein complex is a co-activator for transcription by RNA polymerase II and has various activities including acetylation and deubuiqitination of histones and recruitment of TATA binding protein to promoters. The Spt7p subunit is subject to proteolytic cleavage at its C-terminus resulting in removal of the Spt8p binding domain and generation of the SAGA-related SLIK protein complex. Here, we report identification of the protease responsible for this cleavage. Screening of a protease knockout collection revealed PEP4 to be required for cleavage of Spt7p within SAGA in vitro. Endogenous formation of truncated Spt7p was abolished in cells lacking PEP4. Purified Pep4p but not catalytic dead mutant Pep4p or unrelated Prc1p protease specifically cleaved Spt7p within SAGA into SLIK-related Spt7p. Interestingly, SAGA lacking Spt8p was more sensitive to Pep4p-mediated truncation of Spt7p, suggesting that Spt8p counteracted its own release from SAGA. Strains mimicking constitutive SLIK formation showed increased resistance to rapamycin treatment, suggesting a role for SLIK in regulating cellular responses to nutrient stress.

Status: Published Type: Journal Article PubMed ID: 20498363

Topics addressed in this paper

Number of different genes curated to this paper: 6

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
GCN5 PEP4 PRB1 PRC1 SPT7 SPT8
Additional Literature blue ball blue ball
Function/Process blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball blue ball
Techniques and Reagents blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: