Li DQ, et al. (2010) Inhibition of three selected beverage extracts on alpha-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection. J Agric Food Chem 58(11):6608-13
Abstract: Inhibition of alpha-glucosidase is a therapeutic approach for diabetes. In this study, a method based on online liquid chromatography-diode array detection-tandem mass spectrometry and biochemical detection (LC-DAD-MS/MS-BCD) was developed to screen and identify alpha-glucosidase inhibitors from selected beverage extracts, including pu-erh tea ( Camellia sinensis var. assamica), eagle tea ( Litsea coreana Levl.), and radix glycyrrhizae ( Glycyrrhiza uralensis Fisch.). As a result, two components, (-)-epigallocatechingallate (EGCG) and (-)-epicatechingallate (ECG), as potent alpha-glucosidase inhibitors, were found in pu-erh tea. The IC(50) values of EGCG and ECG on alpha-glucosidase (EC 220.127.116.11, from Saccharomyces cerevisiae ) were 175.1 and 246.9 muM, respectively, and both were lower than that of acarbose (IC(50) = 3553.0 muM), a commercial alpha-glucosidase inhibitor. Kinetic studies revealed that both EGCG and ECG inhibited alpha-glucosidase activity in a noncompetitive manner. The study suggests that the developed LC-DAD-MS/MS-BCD system is a powerful tool for rapid screening and identification of alpha-glucosidase inhibitors in complex samples and that EGCG and ECG may be good candidates as alpha-glucosidase inhibitors.
|Status: Published||Type: Journal Article||PubMed ID: 20443596|
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