Spanova M, et al. (2010) Effect of Lipid Particle Biogenesis on the Subcellular Distribution of Squalene in the Yeast Saccharomyces cerevisiae. J Biol Chem 285(9):6127-33
Abstract: Squalene belongs to the group of isoprenoids and is a precursor for the synthesis of sterols, steroids and ubiquinons. In the yeast Saccharomyces cerevisiae the amount of squalene can be increased by variation of growth conditions or by genetic manipulation. In this report we show that a hem1Delta mutant accumulated a large amount of squalene which was stored almost exclusively in cytoplasmic lipid particles/droplets. Interestingly, a strain bearing a hem1Delta deletion in a dga1Delta lro1Deltaare1Deltaare2Delta quadruple mutant background (QMhem1Delta) which is devoid of the classical storage lipids, triacylglycerols (TAG) and steryl esters (SE), and lacks lipid particles accumulated squalene at similar amounts as the hem1Delta mutant in wild type background. In QMhem1Delta, however, substantial amounts of squalene were found in cellular membranes, especially in microsomes and in the plasma membrane. The fact that QMhem1Delta did not form lipid particles indicated that accumulation of squalene solely was not sufficient to initiate proliferation of lipid particles. Most importantly, these results also demonstrated that (i) squalene was not lipotoxic under the conditions tested; and (ii) organelle membranes in yeast can accommodate relatively large quantities of this non-polar lipid without compromising cellular functions. In summary, localization of squalene as described here can be regarded as an unconventional example of non-polar lipid storage in cellular membranes.
|Status: Published||Type: Journal Article||PubMed ID: 20032462|
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