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Batisse J, et al.  (2009) Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem 284(50):34911-7

Abstract: Nascent mRNAs produced by transcription in the nucleus are subsequently processed and packaged into mRNA ribonucleoprotein particles (mRNPs) before export to the cytoplasm. Here, we have used the poly(A)-binding protein Nab2 to isolate mRNPs from yeast under conditions that preserve mRNA integrity. Upon Nab2-TAP affinity-purification, several mRNA export factors were co-enriched (Yra1, Mex67, THO-TREX) that were present in mRNPs of different size and mRNA length. High-throughput sequencing of the co-precipitated RNAs indicated that Nab2 is associated with the bulk of yeast transcripts with no specificity for different mRNA classes. Electron microscopy revealed that many of the mRNPs have a characteristic elongated structure. Our data suggest that mRNPs although associated with different mRNAs have a unifying core structure.

Status: Published Type: Journal Article PubMed ID: 19840948

Topics addressed in this paper

Number of different genes curated to this paper: 18

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Topics Genes linked to topics (#1 - 10 )
AGA1 CCW12 CTF19 HIF1 HPR1 HYP2 MEX67 MLP1 NAB2 PAB1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#11 - 18 )
PRD1 PWP2 RAD9 SUB2 TEM1 THO2 URA1 YRA1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball
Strains/Constructs blue ball blue ball
Transcription blue ball blue ball blue ball blue ball blue ball

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