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Ear PH and Michnick SW  (2009) A general life-death selection strategy for dissecting protein functions. Nat Methods 6(11):813-6

Abstract: Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.

Status: Published Type: Journal Article PubMed ID: 19820714

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FCY1 MBP1 SKN7 SSK1 SWI4 SWI6 YPD1
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball
Mutants/Phenotypes blue ball blue ball
Primary Literature blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Techniques and Reagents yg ball

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