SGD Paper Help


Page Contents:
Pondugula S, et al.  (2009) Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins. Mol Cell Biol 29(18):4891-905

Abstract: Cells devote considerable resources to nutrient homeostasis, involving nutrient surveillance, acquisition and storage at physiologically relevant concentrations. Many S. cerevisiae transcripts coding for proteins with nutrient uptake functions exhibit peak periodic accumulation during M phase, indicating that an important aspect of nutrient homeostasis involves transcriptional regulation. Inorganic phosphate is a central macronutrient that we previously have shown oscillates inversely with mitotic activation of PHO5. The mechanism of this periodic cell cycle expression remains unknown. To date, only two sequence-specific activators, Pho4 and Pho2, were known to induce PHO5 transcription. Herein, we provide evidence that Mcm1, a MADS-box protein, is essential for PHO5 mitotic activation. In addition, we found that cells simultaneously lacking the forkhead proteins, Fkh1 and Fkh2, exhibited a 2.5-fold decrease in PHO5 expression. The Mcm1-Fkh2 complex, first shown to transactivate genes within the CLB2 cluster that drive G2/M progression, also associated directly at the PHO5 promoter in a cell cycle-dependent manner in chromatin immunoprecipitation assays. Sds3, a component specific to the Rpd3L histone deacetylase complex, was also recruited to PHO5 in G1. These findings provide: i) further mechanistic insight into PHO5 mitotic activation; ii) demonstrate that Mcm1-Fkh2 can function combinatorially with other activators to yield late M/G1 induction and iii) couple the mitotic cell cycle progression machinery to cellular phosphate homeostasis.

Status: Published Type: Journal Article PubMed ID: 19596791

Topics addressed in this paper

Number of different genes curated to this paper: 10

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
CDC15 CDC20 CLB2 CTS1 FKH1 FKH2 MCM1 PHO4 PHO5 SDS3
Additional Literature blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball
Function/Process blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Processing/Modification/Regulation blue ball
Protein-Nucleic Acid Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Regulation of blue ball blue ball blue ball
Regulatory Role blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Transcription blue ball blue ball blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: