Gradolatto A, et al. (2009) A noncanonical bromodomain in the AAA ATPase protein Yta7 directs chromosomal positioning and barrier chromatin activity. Mol Cell Biol 29(17):4604-11
Abstract: Saccharomyces cerevisiae Yta7 is a barrier active protein that modulates transcriptional states at the silent mating locus, HMR. Additionally, Yta7 regulates histone gene transcription and has overlapping functions with known histone chaperones. This study focused on deciphering the functional role of the noncanonical Yta7 bromodomain. Using genetic and epistasis analyses, the Yta7 bromodomain was shown to be necessary for barrier activity at HMR and to have overlapping functions with histone regulators (Asf1, Spt16). Canonical bromodomains can bind to acetylated lysines on histones; however, the Yta7 bromodomain showed an association with histones that was posttranslational modification independent. Further investigation showed that regions of Yta7 other than the bromodomain conferred histone association. ChIP-chip analyses revealed that the Yta7 bromodomain was not solely responsible for histone association, but was necessary for proper chromosomal positioning of Yta7. This work demonstrates that the Yta7 bromodomain engages histones for certain cellular functions like barrier chromatin maintenance and particular Spt16/Asf1 cellular pathways of chromatin regulation.
|Status: Published||Type: Journal Article||PubMed ID: 19581291|
Topics addressed in this paper
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|Genomic co-immunoprecipitation study|
|Protein Sequence Features|
|Protein-Nucleic Acid Interactions|