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Zaborske JM, et al.  (2009) Genome-wide Analysis of tRNA Charging and Activation of the eIF2 Kinase Gcn2p. J Biol Chem 284(37):25254-67

Abstract: When cells are subjected to nutritional stress, uncharged tRNAs accumulate and activate Gcn2p phosphorylation of eIF2 and the general amino acid control pathway. The Gcn2p regulatory domain homologous to histidyl-tRNA synthetases is proposed to bind to uncharged tRNA, directly contributing to activation of Gcn2p. Here we apply a microarray technology to analyze genome-wide changes in tRNA charging in yeast upon activation of Gcn2p in response to amino acid starvation and high salinity, a stress not directly linked to nutritional deficiency. This microarray technology is applicable for all eukaryotic cells. Strains were starved for histidine, leucine or tryptophan and shown to rapidly induce Gcn2p phosphorylation of eIF2. The relative charging level of all tRNAs was measured before and after starvation, and Gcn2p activation and the intracellular levels of the starved amino acid correlate with the observed decrease in tRNA charging. Interestingly, in some cases tRNAs not charged with the starved amino acid became deacylated more rapidly than tRNAs charged with the starved amino acid. This increase in uncharged tRNA levels occurred even though the intracellular levels for these non-starved amino acids remained unchanged. Additionally, treatment of a wild-type strain with high salinity stress showed transient changes in the charging of several different tRNAs. These results suggest that Gcn2p can be activated by many different tRNA species in the cell. These results also depict a complex cellular relationship between tRNA charging, amino acid availability and non-nutrient stress. These relationships are best revealed by simultaneous monitoring of the charging level of all tRNAs.

Status: Published Type: Journal Article PubMed ID: 19546227

Topics addressed in this paper

Number of different genes curated to this paper: 81

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
GCN2 SOE1 SUI2 SUP51 SUP53 SUP54 SUP56 tC(GCA)B tC(GCA)G tC(GCA)P1
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Alias blue ball
Mutants/Phenotypes blue ball
Omics yg ball
Other genomic analysis yg ball
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Protein Processing/Modification/Regulation blue ball
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Regulatory Role blue ball
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Substrates/Ligands/Cofactors blue ball

Topics Genes linked to topics (#11 - 20 )
tC(GCA)P2 tC(GCA)Q tD(GUC)B tD(GUC)D tD(GUC)G1 tD(GUC)G2 tD(GUC)I1 tD(GUC)I2 tD(GUC)J1 tD(GUC)J2
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RNA Levels and Processing blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
tD(GUC)J3 tD(GUC)J4 tD(GUC)K tD(GUC)L1 tD(GUC)L2 tD(GUC)M tD(GUC)N tD(GUC)O tD(GUC)Q tE(UUC)B
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Topics Genes linked to topics (#31 - 40 )
tE(UUC)C tE(UUC)E1 tE(UUC)E2 tE(UUC)E3 tE(UUC)G2 tE(UUC)G3 tE(UUC)I tE(UUC)J tE(UUC)K tE(UUC)L
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Topics Genes linked to topics (#41 - 50 )
tE(UUC)M tE(UUC)P tH(GUG)E1 tH(GUG)E2 tH(GUG)G1 tH(GUG)G2 tH(GUG)H tH(GUG)K tH(GUG)M tH(GUG)Q
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RNA Levels and Processing blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#51 - 60 )
tL(CAA)D tL(CAA)G1 tL(CAA)G3 tL(CAA)K tL(CAA)L tL(CAA)M tL(CAA)N tL(GAG)G tL(UAA)B1 tL(UAA)B2
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Topics Genes linked to topics (#61 - 70 )
tL(UAA)D tL(UAA)K tL(UAA)L tL(UAA)N tL(UAG)J tL(UAG)L1 tL(UAG)L2 tM(CAU)Q2 tR(ACG)D tR(ACG)E
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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RNA Levels and Processing blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#71 - 80 )
tR(ACG)J tR(ACG)K tR(ACG)L tR(ACG)O tW(CCA)G1 tW(CCA)G2 tW(CCA)J tW(CCA)K tW(CCA)M tW(CCA)P
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Regulation of blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
RNA Levels and Processing blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#81 )
tW(UCA)Q
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Regulation of blue ball
RNA Levels and Processing blue ball
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