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Saeki Y, et al.  (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13

Abstract: The 26S proteasome is a highly conserved multisubunit protease that degrades ubiquitinated proteins in eukaryotic cells. The 26S proteasome consists of the proteolytic core particle (CP) and one or two 19S regulatory particles (RPs). Although the mechanisms of CP assembly are well described, the mechanism of RP assembly is largely unknown. Here, we show that four proteasome-interacting proteins (PIPs), Nas2/p27, Nas6/gankyrin, Rpn14/PAAF1, and Hsm3/S5b, bind specific Rpt subunits of the RP and interact each other genetically. Lack of these PIPs resulted in defective assembly of the 26S proteasome at an early stage, suggesting that these proteins are bona fide RP chaperones. Each of the RP chaperones formed distinct specific subassemblies of the base components and escorted them to mature RPs. Our results indicate that the RP assembly is a highly organized and elaborate process orchestrated by multiple proteasome-dedicated chaperones.

Status: Published Type: Journal Article PubMed ID: 19446323

Topics addressed in this paper

Number of different genes curated to this paper: 25

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Topics Genes linked to topics (#1 - 10 )
HSM3 NAS2 NAS6 PRE9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14
Additional Literature blue ball blue ball blue ball blue ball
Cellular Location blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball
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Non-Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball
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Protein Sequence Features blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#11 - 20 )
RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cellular Location blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball

Topics Genes linked to topics (#21 - 25 )
RPT4 RPT5 RPT6 SEM1 UBP6
Additional Literature blue ball blue ball blue ball blue ball blue ball
Protein Sequence Features blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball

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