Lee SY, et al. (2009) pH-Dependent surface-enhanced resonance Raman scattering of yeast iso-1-cytochrome c adsorbed on silver nanoparticle surfaces under denaturing conditions at pH < 3. BMB Rep 42(4):223-6
Abstract: We measured the pH-induced spectral changes of yeast iso-1-cytochrome c on silver nanoparticle surfaces using surface-enhanced resonance Raman scattering (SERRS) at 457.9 nm. At a pH of 3, the Met80 ligand in yeast iso-1-cytochrome c is assumed to dissociate, leading to a marked conformational change as evidenced by the vibrational spectral shifts. The Soret band at 410 nm in the UV-Vis spectrum shifted to 396 nm at pH 3, indicating a transition from a low spin state to a high spin state from a weak interaction with a water molecule. Thus, SERRS spectroscopy can measure the pH-induced denaturalization of cyt c adsorbed on metal nanoparticle surfaces at a lower concentration with a better sensitivity than ordinary resonance Raman spectroscopy. [BMB reports 2009; 42(4): 223-226].
|Status: Published||Type: Journal Article||PubMed ID: 19403046|
Topics addressed in this paper
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|Protein Physical Properties|
|Protein Sequence Features|