Deffieu M, et al. (2009) Glutathione participates in the regulation of mitophagy in yeast. J Biol Chem 284(22):14828-37
Abstract: The anti-oxidant N-acetyl L-cysteine prevented the autophagy-dependent delivery of mitochondria to the vacuoles, as examined by fluorescence microscopy of mitochondria-targeted GFP, transmission electron microscopy and western-blot analysis of mitochondrial proteins. The effect of N-acetyl L-cysteine was specific to mitochondrial autophagy (mitophagy). Indeed, autophagy-dependent activation of alkaline phosphatase and the presence of hallmarks of non-selective microautophagy were not altered by N-acetyl L-cysteine. The effect of N-acetyl L-cysteine was not related to its scavenging properties, but rather to its fuelling effect of glutathione pool. As a matter of fact, the decrease of glutathione pool induced by chemical or genetical manipulation did stimulate mitophagy but not general autophagy. Conversely, the addition of a cell-permeable form of glutathione inhibited mitophagy. Inhibition of glutathione synthesis had no effect in the strain Duth1, that is deficient in selective mitochondrial degradation. These data show that mitophagy can be regulated independently of general autophagy, and that its implementation may depend on the cellular redox status.
|Status: Published||Type: Journal Article||PubMed ID: 19366696|
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