Morel M, et al. (2009) AtHMA3, a P1B-ATPase Allowing Cd/Zn/Co/Pb Vacuolar Storage in Arabidopsis. Plant Physiol 149(2):894-904
Abstract: The Arabidopsis thaliana AtHMA3 protein belongs to the P1B-2 subgroup of the P-type ATPase family, which is involved in heavy metal transport. In a previous study, we have shown, using heterologous expression in the yeast Saccharomyces cerevisiae, that in the presence of toxic metals, AtHMA3 was able to phenotypically complement the Cd/Pb hypersensitive strain ycf1, but not the Zn hypersensitive strain zrc1. In the present study, we demonstrate that AtHMA3 in planta is located to the vacuolar membrane, with a high expression level in guard cells, hydathodes, vascular tissues and the root apex. Confocal imaging in the presence of the Zn/Cd fluorescent probe BTC-5N revealed that AtHMA3 participates in the vacuolar storage of Cd. A T-DNA insertional mutant was found more sensitive to Zn and Cd. Conversely, ectopic overexpression of AtHMA3 improved plant tolerance to Cd, Co, Pb, and Zn; Cd accumulation increased by about two to three-fold in plants overexpressing AtHMA3 compared to wild-type plants. Thus, AtHMA3 likely plays a role in the detoxification of biological (Zn) and non biological heavy metals (Cd, Co and Pb) by participating in their vacuolar sequestration, an original function for a P1B-2 ATPase in a multicellular eukaryote.
|Status: Published||Type: Journal Article||PubMed ID: 19036834|
Topics addressed in this paper
Number of different genes curated to this paper: 2
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.