Peisker K, et al. (2008) Ribosome-associated complex binds to ribosomes in close proximity of Rpl31 at the exit of the polypeptide tunnel in yeast. Mol Biol Cell 19(12):5279-88
Abstract: Monitoring Editor: Jonathan S. Weissman Ribosome-associated complex (RAC) consists of the Hsp40 homolog Zuo1 and the Hsp70 homolog Ssz1. The chaperone participates in the biogenesis of newly synthesized polypeptides. Here we have identified yeast Rpl31, a component of the large ribosomal subunit, as a contact point of RAC at the polypeptide tunnel exit. Rpl31 is encoded by RPL31a and RPL31b, two closely related genes. Deltarpl31aDeltarpl31b displayed slow growth, and sensitivity to low as well as high temperatures. In addition, Deltarpl31aDeltarpl31b was highly sensitive towards aminoglycoside antibiotics and suffered from defects in translational fidelity. With the exception of sensitivity at elevated temperature, the phenotype resembled yeast strains lacking one of the RAC subunits or Rpl39, another protein localized at the tunnel exit. Defects of Deltarpl31aDeltarpl31bDeltazuo1 did not exceed that of Deltarpl31aDeltarpl31b or Deltazuo1. However, the combined deletion of RPL31a, RPL31b, and RPL39 was lethal. Moreover, RPL39 was a multicopy suppressor while overexpression of RAC failed to rescue growth defects of Deltarpl31aDeltarpl31b. The findings are consistent with a model in that Rpl31 and Rpl39 independently affect a common ribosome function, while Rpl31 and RAC are functionally interdependent. Rpl31, while not essential for binding of RAC to the ribosome, might be involved in proper function of the chaperone complex.
|Status: Published||Type: Journal Article||PubMed ID: 18829863|
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