Goldshmidt H, et al. (2008) Role of Protein Translocation Pathways across the Endoplasmic Reticulum in Trypanosoma brucei. J Biol Chem 283(46):32085-98
Abstract: The translocation of secretory and membrane proteins across the ER membrane is mediated by co-translational (via the signal-recognition particle, SRP) and post-translational mechanisms. In this study, we investigated the relative contributions of these two pathways in trypanosomes. A homologue of SEC71, which functions in the post-translocation chaperone pathway in yeast, was identified and silenced by RNAi. This factor is essential for parasite viability. In SEC71 silenced cells, signal peptide (SP)-containing proteins traversed the ER but several were mislocalized, whereas polytopic membrane protein biogenesis was unaffected. Surprisingly, trypanosomes can interchangeably utilize two of the pathways to translocate SP-containing proteins, except for glycosyl-phosphatidyl-inositol (GPI)-anchored proteins, whose level was reduced in SEC71-silenced cells but not in cells depleted for SRP68, an SRP-binding protein. Entry of SP- containing proteins to the ER was significantly blocked only in cells co-silenced for the two translocation pathways (SEC71 and SRP68). SEC63, a factor essential for both translocation pathways in yeast, was identified and silenced by RNAi. SEC63 silencing affected entry to the ER of both SP-containing proteins and polytopic membrane proteins, suggesting that, as in yeast, this factor is essential for both translocation pathways in vivo. This study suggests that unlike bacteria or other eukaryotes, trypanosomes are generally promiscuous in their choice of mechanism for translocating SP-containing proteins to the ER, although the SRP-independent pathway is favored for GPI-anchored proteins, which are the most abundant surface proteins in these parasites.
|Status: Published||Type: Journal Article||PubMed ID: 18768469|
Topics addressed in this paper
Number of different genes curated to this paper: 2
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