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de Groot PW, et al.  (2001) A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae. Comp Funct Genomics 2(3):124-42

Abstract: Using a hierarchical approach, 620 non-essential single-gene yeast deletants generated by EUROFAN I were systematically screened for cell-wall-related phenotypes. By analyzing for altered sensitivity to the presence of Calcofluor white or SDS in the growth medium, altered sensitivity to sonication, or abnormal morphology, 145 (23%) mutants showing at least one cell wall-related phenotype were selected. These were screened further to identify genes potentially involved in either the biosynthesis, remodeling or coupling of cell wall macromolecules or genes involved in the overall regulation of cell wall construction and to eliminate those genes with a more general, pleiotropic effect. Ninety percent of the mutants selected from the primary tests showed additional cell wall-related phenotypes. When extrapolated to the entire yeast genome, these data indicate that over 1200 genes may directly or indirectly affect cell wall formation and its regulation. Twenty-one mutants with altered levels of beta1,3-glucan synthase activity and five Calcofluor white-resistant mutants with altered levels of chitin synthase activities were found, indicating that the corresponding genes affect beta1,3-glucan or chitin synthesis. By selecting for increased levels of specific cell wall components in the growth medium, we identified 13 genes that are possibly implicated in different steps of cell wall assembly. Furthermore, 14 mutants showed a constitutive activation of the cell wall integrity pathway, suggesting that they participate in the modulation of the pathway either directly acting as signaling components or by triggering the Slt2-dependent compensatory mechanism. In conclusion, our screening approach represents a comprehensive functional analysis on a genomic scale of gene products involved in various aspects of fungal cell wall formation.

Status: Published Type: Journal Article PubMed ID: 18628907

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AAD4 AIM22 ALG12 ALK2 ARE2 ARG2 ARP5 ASI2 ATG4 ATG9
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AVL9 AVT1 AVT4 BFA1 BNI4 BNI5 BRE1 BRE4 BRE5 BUD20
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Topics Genes linked to topics (#21 - 30 )
BUD32 CAF120 CAF40 CNM67 CTK2 CWC23 CYK3 DAS1 DBP3 DLS1
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Topics Genes linked to topics (#31 - 40 )
ECM33 ELP2 EOS1 ERG4 FIG4 FPK1 FRE7 FTH1 GET3 GLT1
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Topics Genes linked to topics (#41 - 50 )
GPI1 GPI14 GRE2 HNT1 HOC1 HSL7 IES2 INP52 ITC1 IWR1
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Topics Genes linked to topics (#51 - 60 )
IZH3 JJJ1 JLP1 KHA1 LAS21 LCB3 LDB17 LDB19 LDB7 LEM3
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Topics Genes linked to topics (#61 - 70 )
LIA1 LSG1 LSM1 MDS3 MED2 MOG1 MPS3 MRPL22 MRPS18 MTC4
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Topics Genes linked to topics (#71 - 80 )
NCL1 NCS2 NRP1 OCA6 PAA1 PAP2 PEX17 PHO23 PIN4 POL32
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Topics Genes linked to topics (#81 - 90 )
PTK2 RAD59 RCY1 RFM1 RIM20 RIM21 ROT2 RRG1 RRG9 RRN10
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Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#91 - 100 )
RTC1 RTT101 RTT102 SEH1 SGT2 SHG1 SHS1 SLM6 SLT2 SNF11
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#101 - 110 )
SNT2 SQS1 STB1 SWD3 SYS1 TCM62 THP1 TLG2 TOS1 TRM10
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Topics Genes linked to topics (#111 - 120 )
TRM11 TRM3 UBP13 UBR2 VAC7 VAM6 VNX1 VPS13 VPS53 VPS55
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Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#121 - 130 )
XYL2 YAF9 YBL029W YBR016W YBR071W YDL124W YDL158C YDR015C YGL114W YGL138C
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Topics Genes linked to topics (#131 - 140 )
YGR210C YJL018W YJL070C YLL044W YNL058C YNL095C YNL134C YNR048W YNR068C YOL036W
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Topics Genes linked to topics (#141 - 146 )
YPC1 YPQ1 YPT10 YPT11 YRR1 ZAP1
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Function/Process blue ball blue ball blue ball blue ball blue ball blue ball
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