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Niu W, et al.  (2008) Mechanisms of Cell Cycle Control Revealed by a Systematic and Quantitative Overexpression Screen in S. cerevisiae. PLoS Genet 4(7):e1000120

Abstract: Regulation of cell cycle progression is fundamental to cell health and reproduction, and failures in this process are associated with many human diseases. Much of our knowledge of cell cycle regulators derives from loss-of-function studies. To reveal new cell cycle regulatory genes that are difficult to identify in loss-of-function studies, we performed a near-genome-wide flow cytometry assay of yeast gene overexpression-induced cell cycle delay phenotypes. We identified 108 genes whose overexpression significantly delayed the progression of the yeast cell cycle at a specific stage. Many of the genes are newly implicated in cell cycle progression, for example SKO1, RFA1, and YPR015C. The overexpression of RFA1 or YPR015C delayed the cell cycle at G2/M phases by disrupting spindle attachment to chromosomes and activating the DNA damage checkpoint, respectively. In contrast, overexpression of the transcription factor SKO1 arrests cells at G1 phase by activating the pheromone response pathway, revealing new cross-talk between osmotic sensing and mating. More generally, 92%-94% of the genes exhibit distinct phenotypes when overexpressed as compared to their corresponding deletion mutants, supporting the notion that many genes may gain functions upon overexpression. This work thus implicates new genes in cell cycle progression, complements previous screens, and lays the foundation for future experiments to define more precisely roles for these genes in cell cycle progression.

Status: Published Type: Journal Article PubMed ID: 18617996

Topics addressed in this paper

Number of different genes curated to this paper: 119

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ABF1 ACT1 AIM20 ALG6 AME1 ARC1 ARF1 ASK1 ATG26 AVO2
Additional Literature blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Large-scale phenotype analysis yg ball
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Omics yg ball
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Topics Genes linked to topics (#11 - 20 )
BET4 BUL1 CBF1 CDC31 CDC39 CLB2 CLB3 CLB5 CLB6 CLN1
Additional Literature blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#21 - 30 )
CRT10 CST6 CST9 CYT1 DHR2 ENO2 ENT3 FAR1 FRM2 FRS2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
FTR1 GAT4 GEA2 GOS1 GPT2 HEL2 HHO1 HOS3 IES3 IME2
Additional Literature blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#41 - 50 )
IMG1 KAR1 KAR4 LEU5 MAD2 MIG3 MNN10 MRH1 MSN5 MTH1
Additional Literature blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#51 - 60 )
MVB12 MYO2 MZM1 NCB2 NHP10 NIP100 NTH1 PAC2 PBS2 PDR17
Additional Literature blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
PMT5 PPZ1 PRP31 PRR2 RAD9 RDL2 RFA1 RLI1 RPA14 RPC82
Additional Literature blue ball blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#71 - 80 )
RPL14B RPS26B SAN1 SEC17 SET3 SGF73 SGN1 SGT2 SHE1 SHY1
Additional Literature blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#81 - 90 )
SKO1 SLK19 SPC2 SPC97 SPO13 SPO77 STE11 STE2 STE20 STE4
Additional Literature blue ball blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball
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Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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  • To go to the Locus page for a gene, click on the gene name.

Topics Genes linked to topics (#91 - 100 )
STE5 SUR7 TEA1 TEC1 TFG2 TMA64 TPM2 TRM5 TUB2 URN1
Additional Literature blue ball blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#101 - 110 )
VAS1 VTC4 WSC2 YAP1 YBR131C-A YDL159W-A YGR109W-A YHR131C YHR177W YIR016W
Additional Literature blue ball blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#111 - 119 )
YJL012C-A YJL077W-A YLL066W-B YLR123C YLR149C YML053C YOR131C YPL247C YPR015C
Additional Literature blue ball blue ball
Cell Cycle Phase Involved blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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