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Li Z, et al.  (2008) Cdc34p Ubiquitin-Conjugating Enzyme Is a Component of the Tombusvirus Replicase Complex and Ubiquitinates p33 Replication Protein. J Virol 82(14):6911-26

Abstract: To identify host proteins interacting with Tomato bushy stunt virus (TBSV) replication proteins in a genome-wide scale, we have used the yeast proteome microarray carrying 4088 purified proteins. This approach led to the identification of 58 yeast proteins that interacted with p33 replication protein. The identified host proteins included protein chaperones, ubiquitin-associated proteins, translation factors, RNA modifying enzymes and other proteins with yet unknown functions. We confirmed that 19 of the identified host proteins bound to p33 in vitro or in a split-ubiquitin-based two-hybrid assay. Further analysis of Cdc34p E2 ubiquitin-conjugating enzyme, which is one of the host proteins interacting with p33, revealed that Cdc34p is a novel component of the purified viral replicase. Down-regulation of Cdc34p expression in yeast, which supports replication of a TBSV replicon (rep)RNA, reduced repRNA accumulation and the activity of the tombusvirus replicase by up to 5-fold. Over-expression of wt, but not an E2 defective mutant of Cdc34p, increased repRNA accumulation, suggesting significant role for the ubiquitin-conjugating enzyme function of Cdc34p in TBSV replication. Also, Cdc34p was able to ubiquitinate p33 in vitro. In addition, we have shown that p33 becomes ubiquitinated in vivo. We propose that ubiquitination of p33 likely alters its function or affects the recruitment of host factors during TBSV replication.

Status: Published Type: Journal Article PubMed ID: 18463149

Topics addressed in this paper

Number of different genes curated to this paper: 69

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ALA1 ARP8 BFR1 BUD21 CDC34 DBP3 DDR48 DOT1 EFB1 ELF1
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Large-scale protein interaction yg ball
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Omics yg ball
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Topics Genes linked to topics (#11 - 20 )
EMI2 ERB1 ERR2 FMP40 FOX2 GIM3 GPH1 GSY2 HBS1 HOR2
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Topics Genes linked to topics (#21 - 30 )
IPI3 ISN1 IWR1 JJJ1 JJJ3 LOA1 MAM33 MAP1 NAP1 NOB1
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Topics Genes linked to topics (#31 - 40 )
NPL3 OTU2 PDI1 PLP2 POL30 PYC1 QCR6 RDS2 RIB2 RPL8A
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Topics Genes linked to topics (#41 - 50 )
RSP5 RTT106 SAS10 SHO1 SMK1 SPT16 STM1 TDA3 TEF2 TIF1
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Topics Genes linked to topics (#51 - 60 )
TIF11 TOM71 TRM1 TRZ1 TSR2 TUM1 UBA1 UBP10 UBP15 URN1
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Topics Genes linked to topics (#61 - 69 )
UTP7 YCR016W YDR161W YGL242C YGR017W YGR027W-A YKL023W YLR125W YOR309C
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