Seong C, et al. (2008) Molecular Anatomy of the Recombination Mediator Function of Saccharomyces cerevisiae Rad52. J Biol Chem 283(18):12166-74
Abstract: A helical filament of Rad51 on ssDNA, called the presynaptic filament, catalyzes DNA joint formation during homologous recombination. Rad52 facilitates presynaptic filament assembly, and this recombination mediator activity is thought to rely on Rad52's interactions with Rad51, the ssDNA binding protein RPA, and ssDNA. The N-terminal region of Rad52, which has DNA binding activity and an oligomeric structure, is thought to be crucial for mediator activity and recombination. Unexpectedly, we find that the carboxyl-terminal region of Rad52 also harbors a DNA binding function. Importantly, the Rad52 C-terminal portion alone can promote Rad51 presynaptic filament assembly. The middle portion of Rad52 associates with DNA-bound RPA and contributes to the recombination mediator activity. Accordingly, expression of a protein species that harbors the middle and C-terminal regions of Rad52 in the rad52 327 background enhances the association of Rad51 protein with a HO-made DNA double-strand break and partially complements the MMS sensitivity of the mutant cells. Our results provide a mechanistic framework for rationalizing the multi-faceted role of Rad52 in recombination and DNA repair.
|Status: Published||Type: Journal Article||PubMed ID: 18310075|
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