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Kim HJ, et al.  (2004) A yeast DNA microarray for the evaluation of toxicity in environmental water containing burned ash. Environ Monit Assess 92(1-3):253-72

Abstract: Numerous studies on the hazard assessment and epidemiological health responses to burned ash have been reported. However, there is little information on the potential toxicity of unknown chemical complexes in burned ash. For an overall evaluation of the multiple toxicities of burned ash, a DNA microarray was used in this study, as a new attempt to assess these toxicities. Using the global gene expression on yeast DNA chip to reflect the changes in mRNA levels, our study discovered a lot of evidences for the action of cell homeostasis and stress response etc., against the toxic effects on yeast cells. On the genes of 5,117 open reading frames (ORFs), as valid spots in a microarray, 997 were up-regulated, 1,259 were down-regulated and 2,861 remained unchanged. A detailed analysis of the microarray revealed the genes that were dynamically correlated to the function of the subcellular localization, energy/metabolism, various stress responses/cell homeostasis and detoxification. Significantly, the toxicities, caused by reactive oxygen species (ROS), metals and the other xenobiotics, were indicated in burned ash. Also, the possibility of mutagenicity of the burned ash was suggested on the basis of the DNA repair related genes.

Status: Published Type: Journal Article PubMed ID: 15038548

Topics addressed in this paper

Number of different genes curated to this paper: 93

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AAH1 ATC1 ATO2 BOP2 CAD1 CCL1 CCP1 CDC42 CRS5 CSR2
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Topics Genes linked to topics (#11 - 20 )
CTA1 CTT1 CUP1-1 CUP1-2 CYB2 DBP3 DDR48 ECM13 ECM4 FAR1
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Topics Genes linked to topics (#21 - 30 )
FDH1 GIS1 GLO1 GPA1 GPX1 GPX2 GRE1 GRX1 GRX2 GSC2
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Alias blue ball
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Topics Genes linked to topics (#31 - 40 )
GSH1 GTT1 GTT2 GUA1 HSP12 HSP26 IMP2 IMP3 JEN1 KRR1
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Topics Genes linked to topics (#41 - 50 )
MF(ALPHA)2 MGT1 MLH3 MMS2 MNR2 MRT4 MSR1 MYO1 NCE103 NIP7
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Topics Genes linked to topics (#51 - 60 )
NOP56 PHR1 PPT1 PWP1 RAD4 RAD51 REG2 RNR4 RPL18B RPL20A
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Topics Genes linked to topics (#61 - 70 )
RPL22A RPL2B RPL30 RPL33A RPL33B RPL9A RPS0A RPS0B RPS12 RPS13
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Topics Genes linked to topics (#71 - 80 )
RPS2 RPS22A RPS7A RPS8A RPS9B SHO1 SNU13 SOD1 SOD2 SSL2
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Topics Genes linked to topics (#81 - 90 )
STE12 STE20 STE3 STL1 SUR4 THI4 TRX2 TSA1 UGX2 WSC2
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Genomic expression study blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#91 - 93 )
XRS2 YPT53 ZWF1
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