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Amaro IA, et al.  (2008) The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex. Genetics 178(2):703-9

Abstract: Stu1 is the S. cerevisiae member of the CLASP family of microtubule plus-end tracking proteins and is essential for spindle formation. A genome-wide screen for gene deletions that are lethal in combination with the temperature-sensitive stu1-5 allele identified ldb18. ldb18 cells exhibit defects in spindle orientation similar to those caused by a block in the dynein pathway. Consistent with this observation, ldb18 is synthetic lethal with mutations affecting the Kar9 spindle orientation pathway, but not with those affecting the dynein pathway. We show that Ldb18 is a component of dynactin, a complex required for dynein activity in yeast and mammalian cells. Ldb18 shares modest sequence and structural homology with the mammalian dynactin component p24. It interacts with dynactin proteins in two-hybrid and co-immunoprecipitation assays, and co-migrates with them as a 20 S complex during sucrose gradient sedimentation. In ldb18 cells, the interaction between Nip100 (p150Glued) and Jnm1 (dynamitin) is disrupted, while the interaction between Jmn1 and Arp1 is not affected. These results indicate that p24 is required for attachment of the p150Glued arm to dynamitin and the remainder of the dynactin complex. The genetic interaction of ldb18 with stu1-5 also supports the notion that dynein/dynactin helps to generate a spindle pole separating force.

Status: Published Type: Journal Article PubMed ID: 18245366

Topics addressed in this paper

Number of different genes curated to this paper: 49

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
AIM4 APQ12 ARP1 ARP10 ASE1 BIK1 BIM1 BNI1 BUB1 BUB3
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Topics Genes linked to topics (#11 - 20 )
CHL4 CIN1 CIN2 CTF18 CTF19 CTF3 CTF8 DYN1 DYN2 DYN3
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Topics Genes linked to topics (#21 - 30 )
GIM3 GIM4 GIM5 IML3 JNM1 KAR9 KIP3 LDB18 MAD1 MAD2
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Topics Genes linked to topics (#31 - 40 )
MAD3 MCM21 MCM22 MOG1 NIP100 NPL3 NUM1 NUP170 NUP188 PAC1
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Topics Genes linked to topics (#41 - 49 )
PAC10 PAC11 PAC2 SAC3 STU1 TEX1 TUB3 VIK1 YKE2
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