SGD Paper Help


Page Contents:
Calvert ME, et al.  (2008) Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol 28(4):1313-25

Abstract: In S. cerevisiae, the evolutionarily conserved nucleocytoplasmic shuttling protein Nap1 is a cofactor for the import of histones H2A and H2B, a chromatin assembly factor and a mitotic factor involved in regulation of bud formation. To understand the mechanism by which Nap1 function is regulated, Nap1-interacting factors were isolated and identified by Mass Spectrometry. Amongst these proteins we identified several kinases, including Casein Kinase 2 (CK2), and a new bud neck associated protein, Nba1. Consistent with our identification of the interacting kinases, we showed that Nap1 is phosphorylated in vivo at eleven sites, and that Nap1 is phosphorylated by CK2 at three substrate serines. Phosphorylation of these serines was not necessary for normal bud formation, but mutation of these serines to either alanine or aspartic acid resulted in cell cycle changes including a prolonged S-phase, suggesting that reversible phosphorylation by CK2 is important for cell cycle regulation. Nap1 can shuttle between the nucleus and cytoplasm, and we also showed that CK2 phosphorylation promotes the import of Nap1 into the nucleus. In conclusion, our data show that Nap1 phosphorylation by CK2 appears to regulate Nap1 localization and is required for normal progression through S phase.

Status: Published Type: Journal Article PubMed ID: 18086883

Topics addressed in this paper

Number of different genes curated to this paper: 6

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Topics not linked to Genes Genes linked to topics
CKA2 CKI1 GIN4 KCC4 NAP1 NBA1
Cell Cycle Phase Involved blue ball
Cellular Location blue ball
Function/Process blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball
Large-scale protein interaction yg ball
Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball blue ball
Omics yg ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball
Protein Processing/Modification/Regulation blue ball
Protein Sequence Features blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball
Regulation of blue ball
Regulatory Role blue ball
Substrates/Ligands/Cofactors blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: