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Suter B, et al.  (2007) Examining protein protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system. Genome Res 17(12):1774-82

Abstract: Comprehensive approaches to detect protein-protein interactions (PPIs) have been most successful in the yeast model system. Here we present "Cross-and-Capture," a novel assay for rapid, sensitive assessment of PPIs via pulldown of differently tagged yeast strain arrays. About 500 yeast genes that function in DNA replication, repair, and recombination and nuclear proteins of unknown function were chromosomally tagged with six histidine residues or triple VSV epitopes. We demonstrate that the assay can interrogate a wide range of previously known protein complexes with increased resolution and sensitivity. Furthermore, we use "Cross-and-Capture" to identify two novel protein complexes: Rtt101p-Mms1p and Sae2p-Mre11p. The Rtt101p-Mms1p interaction was subsequently characterized by genetic and functional analyses. Our studies establish the "Cross-and-Capture" assay as a novel, versatile tool that provides a valuable complement for the next generation of yeast proteomic studies.

Status: Published Type: Journal Article PubMed ID: 17989249

Topics addressed in this paper

Number of different genes curated to this paper: 40

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
ASF1 CAC2 CCL1 CCS1 CDC28 CKA1 CKA2 CKB1 CKB2 CLN3
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Techniques and Reagents yg ball

Topics Genes linked to topics (#11 - 20 )
FOB1 MLH1 MMS1 MMS21 MRE11 ORC1 ORC5 PMS1 PRI1 PRI2
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball blue ball
Protein Physical Properties blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
PSY3 RAD1 RAD10 RAD52 RAD53 RFC3 RFC5 RLF2 RTT101 RTT107
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball
Mutants/Phenotypes blue ball
Primary Literature blue ball blue ball
Protein Processing/Modification/Regulation blue ball
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Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RTT109 SAE2 SAW1 SHU1 SMC5 SOD1 TFB1 VPS75 YKU70 YKU80
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Primary Literature blue ball
Protein Physical Properties blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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