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Swatkoski S, et al.  (2007) Integration of Residue-Specific Acid Cleavage into Proteomic Workflows. J Proteome Res 6(11):4525-4527

Abstract: Microwave-accelerated proteolysis using acetic acid has been shown to occur specifically on either or both sides of aspartate residues. This chemical cleavage is applied to the yeast ribosome proteome to evaluate its suitability for incorporation into high-throughput automated workflows. Peptide product mixtures were analyzed using either an HPLC-ESI-LTQ-Orbitrap or an HPLC-MALDI-TOF2. The peptides were readily identified, using MASCOT with a modified enzyme rule, and provided information about 73% of the proteome. Implications are considered of the extended length and the presence of multiple basic residues in these peptides. Keywords: chemical proteolysis * microwave acceleration * Orbitrap * MASCOT * yeast ribosomes * residue selective cleavage.

Status: Published Type: Journal Article PubMed ID: 17902642

Topics addressed in this paper

Number of different genes curated to this paper: 71

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
RPL10 RPL11A RPL11B RPL12A RPL13B RPL14A RPL14B RPL16A RPL16B RPL19A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Techniques and Reagents yg ball

Topics Genes linked to topics (#11 - 20 )
RPL1A RPL1B RPL20A RPL20B RPL22A RPL23A RPL25 RPL26A RPL26B RPL2A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
RPL3 RPL30 RPL38 RPL4A RPL4B RPL5 RPL6A RPL6B RPL8A RPL8B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
RPL9A RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS10A RPS12 RPS14B
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#41 - 50 )
RPS15 RPS16A RPS17A RPS18A RPS18B RPS19A RPS19B RPS1A RPS2 RPS20
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#51 - 60 )
RPS21B RPS22A RPS22B RPS24A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#61 - 70 )
RPS29B RPS3 RPS4A RPS4B RPS5 RPS7A RPS7B RPS8A RPS8B RPS9A
Additional Literature blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein Physical Properties blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Protein-protein Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#71 )
RPS9B
Additional Literature blue ball
Protein Physical Properties blue ball
Protein-protein Interactions blue ball

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