Nakamata K, et al. (2007) KEG1/YFR042w Encodes a Novel Kre6-binding Endoplasmic Reticulum Membrane Protein Responsible for beta-1,6-Glucan Synthesis in Saccharomyces cerevisiae. J Biol Chem 282(47):34315-24
Abstract: KEG1/YFR042w of Saccharomyces cerevisiae is an essential gene which encodes a 200-amino acid polypeptide with 4 predicted transmembrane domains. The GFP- or 6myc-tagged Keg1 protein showed the typical characteristics of an integral membrane protein and was found in the endoplasmic reticulum (ER) by fluorescence imaging. Immunoprecipitation from the Triton X-100-solubilzed cell lysate revealed that Keg1 binds to Kre6 which has been known to participate in beta-1,6-glucan synthesis. To analyze the essential function of Keg1 in more detail, we constructed temperature-sensitive mutant alleles by error-prone polymerase chain reaction. The keg1-1 mutant cells showed common phenotype with kre6 mutant including hypersensitivity to Calcofluor white, reduced sensitivity to the K1 killer toxin, and reduced content of beta-1,6-glucan in the cell wall. These results suggest that Keg1 and Kre6 have a cooperative role in beta-1,6-glucan synthesis in S. cerevisiae.
|Status: Published||Type: Journal Article||PubMed ID: 17893149|
Topics addressed in this paper
Number of different genes curated to this paper: 2
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