SGD Paper 
Sun M and Fasullo M (2007) Activation of the budding yeast securin Pds1 but not Rad53 correlates with double-strand break-associated G2/M cell cycle arrest in a mec1 hypomorphic mutant. Cell Cycle 6(15):1896-902
Abstract: Budding yeast Mec1, encoded by the yeast ATR/ATM homolog, negatively regulates cell cycle progression by activating Rad53 (Chk2) and Chk1, two parallel downstream checkpoint pathways. Chk1 phosphorylates Pds1 (securin), which prevents Pds1 degradation. We determined whether activation of both downstream pathways is required to establish G(2) arrest in response to double-strand breaks (DSBs). In a hypomorphic mec1 mutant, Rad53 activation was not required to establish G(2) arrest triggered by a single HO endonuclease-generated DSB. However, Pds1 phosphorylation did correlate with G(2) arrest and mec1-21 pds1 cells did not arrest in G(2) after exposure to ionizing radiation. The G(2) checkpoint genes, CHK1 and PDS1, did confer radiation resistance in mec1-21, indicating that CHK1-mediated pathway is functional in the mec1 hypomorph. Thus, phosphorylation of Pds1 but not Rad53 correlates with G(2) arrest in response to DSBs in the mec1 hypomorphic mutant.
| Status: Published | Type: Journal Article | PubMed ID: 17671432 |
Topics addressed in this paper
Number of different genes curated to this paper: 5
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