Kasahara K, et al. (2007) Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae. Mol Cell Biol 27(19):6686-6705
Abstract: HMO1 is a high mobility group B (HMGB) protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation (ChIP) to study the roles of HMO1, FHL1 and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I (Pol I)-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1-abundance at the promoter and the HMO1-dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether or not they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.
|Status: Published||Type: Journal Article||PubMed ID: 17646381|
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