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Smolka MB, et al.  (2007) Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases. Proc Natl Acad Sci U S A 104(25):10364-9

Abstract: Understanding the role of DNA damage checkpoint kinases in the cellular response to genotoxic stress requires the knowledge of their substrates. Here, we report the use of quantitative phosphoproteomics to identify in vivo kinase substrates of the yeast DNA damage checkpoint kinases Mec1, Tel1, and Rad53 (orthologs of human ATR, ATM, and CHK2, respectively). By analyzing 2,689 phosphorylation sites in wild-type and various kinase-null cells, 62 phosphorylation sites from 55 proteins were found to be controlled by the DNA damage checkpoint. Examination of the dependency of each phosphorylation on Mec1 and Tel1 or Rad53, combined with sequence and biochemical analysis, revealed that many of the identified targets are likely direct substrates of these kinases. In addition to several known targets, 50 previously undescribed targets of the DNA damage checkpoint were identified, suggesting that a wide range of cellular processes is likely regulated by Mec1, Tel1, and Rad53.

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't PubMed ID: 17563356

Topics addressed in this paper

Number of different genes curated to this paper: 30

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Topics Topics not linked to Genes Genes linked to topics (#1 - 10 )
CEP3 CPR5 CTF4 DEF1 DUN1 EXO1 HPC2 ITC1 MEC1 MLP1
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Topics Genes linked to topics (#11 - 20 )
NET1 NPL3 NSP1 NUP1 NUP2 NUP60 PGM2 PLM2 RAD53 RAD54
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Topics Genes linked to topics (#21 - 30 )
RNR3 RPA14 SEC31 SHS1 SNF2 TAF2 TEL1 TOF1 USO1 YRB2
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Regulation of blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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