Hu Z, et al. (2007) Genetic reconstruction of a functional transcriptional regulatory network. Nat Genet 39(5):683-687
Abstract: Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.
|Status: Published||Type: Journal Article||PubMed ID: 17417638|
Topics addressed in this paper
Number of different genes curated to this paper: 2
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