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Tekamp PA, et al.  (1980) Transcription and in vitro processing of yeast 5 S rRNA. J Biol Chem 255(19):9501-6

Abstract: A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5'-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3'-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.FAU - Tekamp, P .

Status: Published Type: Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S. PubMed ID: 6251092

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