Malpartida F and Serrano R (1981) Reconstitution of the proton-translocating adenosine triphosphatase of yeast plasma membranes. J Biol Chem 256(9):4175-7
Abstract: The plasma membrane ATPases of eukaryotic cells of the vegetable type (fungi, plants, and algae) have been postulated to operate as proton pumps which generate membrane potentials and drive the uptake of nutrients by proton co-transport (Poole, R. J. (1978) Annu. Rev. Plant Physiol. 29, 437-460). In order to verify this important physiological role, a purified preparation of the yeast plasma membrane ATPase has been reconstituted with soybean phospholipids by a freeze-thaw-sonication procedure. The reconstituted proteoliposomes catalyzed a 32Pi-ATP exchange partially sensitive to proton ionophores (uncouplers) and to the proton-potassium exchange carrier nigericin. The reaction was completely inhibited by the nonspecific ionophore gramicidin and by the combination of uncouplers with the potassium ionophore valinomycin. These results are interpreted as evidence for two types of proton transport catalyzed by the enzyme preparation: electrogenic proton transport and electroneutral proton-potassium exchange.FAU - Malpartida, .
|Status: Published||Type: Journal Article||PubMed ID: 6163779|
Topics addressed in this paper
Number of different genes curated to this paper: 2
- To find other papers on a gene and topic, click on the colored ball in the appropriate box.
- displays other papers with information about that topic for that gene.
- displays other papers in SGD that are associated with that topic.
The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
- To go to the Locus page for a gene, click on the gene name.