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Sharma VM, et al.  (2007) Histone deacetylases RPD3 and HOS2 regulate the transcriptional activation of DNA damage-inducible genes. Mol Cell Biol 27(8):3199-210

Abstract: DNA microarray and genetic studies in Saccharomyces cerevisiae have demonstrated that histone deacetylases (HDACs) are required for transcriptional activation and repression, but the mechanism by which they activate transcription remains poorly understood. We show that two histone deacetylases, RPD3 and HOS2, are required for the activation of the DNA damage inducible genes RNR3 and HUG1. Using mutants specific for the Rpd3L complex, we show that it is responsible for regulating RNR3. Furthermore, unlike what was described at the GAL genes, Rpd3L regulates the activation of RNR3 by deacetylating nucleosomes at the promoter, not the ORF. Rpd3 is recruited to the upstream repression sequences (URS) of RNR3, which, surprisingly, does not require Tup1 or Crt1. Chromatin remodeling and TFIID recruitment is largely unaffected in the Deltarpd3/Deltahos2 mutant, but RNA polymerase II recruitment is strongly reduced, arguing that Rpd3 and Hos2 regulate later stages in preinitiation complex assembly or facilitate multiple rounds of polymerase recruitment. Furthermore, the histone H4 acetyltransferase Esa1 is required for activation of RNR3 and HUG1. Thus, reduced or un-regulated, constitutive histone H4 acetylation is detrimental to promoter activity, suggesting that HDAC-dependent mechanisms are in place to reset promoters to allow high levels of transcription.

Status: Published

Type: Journal Article

PubMed ID: 17296735

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Sharma VM Tomar RS Dempsey AE Reese JC Papers in SGD Colleagues in SGD PubMed Google Scholar

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