SGD Paper 
Sherriff JA, et al. (2007) The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2. Mol Cell Biol 27(8):2848-60
Abstract: Fork head (Fkh) transcription factors influence cell death, proliferation, differentiation and the cell cycle. In yeast, Fkh2 both activates and represses transcription of CLB2 encoding a B-type cyclin. CLB2 is expressed during G2/M and repressed during G1. Fkh2 recruits the co-activator Ndd1, an interaction which is promoted by Clb2/Cdk1-dependent phosphorylation of Ndd1 suggesting that CLB2 is auto-regulated. Ndd1 is proposed to function by antagonizing Fkh2-mediated repression but nothing is known about the mechanism. Here we ask how Fkh2 represses CLB2. We show that Fkh2 controls a repressive chromatin structure that initiates in the early coding region of CLB2 and spreads up the promoter during M and G1. The Isw2 chromatin remodeling ATPase cooperates with Fkh2 to remodel the chromatin and repress CLB2 expression throughout the cell cycle. In addition, the related factors Isw1 and Fkh1 configure the chromatin at the early coding region and negatively regulate CLB2 expression but only during G2/M. Thus the cooperative actions of two fork head transcription factors and two chromatin remodeling ATPases combine to regulate CLB2. We propose that chromatin mediated repression by Isw1 and Isw2 may serve to limit activation of CLB2 expression by the Clb2/Cdk1 kinase during G2/M and to fully repress expression during G1.
| Status: Published | Type: Journal Article | PubMed ID: 17283050 |
Topics addressed in this paper
Number of different genes curated to this paper: 6
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